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Apparent RNA dissociation constants (Kd-values) in electrophoretic gel shift assays of binding of BcBL1 and BcBL2 to PyrR
| BcBL1 |
BcBL2 |
||
|---|---|---|---|
| Kd1 (nM) | Kd2 (nM) | Kd (nM) | |
| No effector | 0.18 ± 0.04 | 7650 ± 2500 | 0.11 ± 0.04 |
| UMP | 0.06 ± 0.02 | 6300 ± 4900 | 0.07 ± 0.01 |
| GMP | 19 ± 8 | 16800 ± 7400 | 3.3 ± 1.9 |
UMP and GMP were present at 0.5 mM. The data are averages of three to four independent determinations.
