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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Cancer Res. 2010 Nov 29;9(1):25–35. doi: 10.1158/1541-7786.MCR-10-0497

Figure 4.

Figure 4

VEGF mediates CAF-induced lung adenocarcinoma cell invasion. A, 344SQ cells were cocultured with CAFs in the presence (+) or absence (−) of anti-VEGF-A neutralizing antibodies. Invasive tumor cells were photographed (images) and counted in at least 5 separate microscopic fields per well, which were averaged, and the mean values per well ± SD were calculated from replicate wells (bar graphs). B, miR-200 suppresses CAF-induced tumor cell invasion. Transwell invasion assays of 344SQ cells that stably express miR-200b (200b) or empty (control) lentiviral vectors in coculture with CAFs. Invaded cells were stained, photographed (image), and counted in at least 5 separate microscopic fields per well, which were averaged, and the mean values per well ± SD were calculated from replicate wells (bar graph). C, The indicated tumor cell lines derived from KrasLA1/+; p53R172HΔG/+ mice were monocultured in the upper chamber of a dual-chamber well, and recombinant VEGF-A was added (+) or not added (−) to the lower chamber. Invasive tumor cells were photographed (images) and counted in at least 5 separate microscopic fields per well, which were averaged, and the mean values per well ± SD were calculated from replicate wells (bar graphs).