. Author manuscript; available in PMC: 2011 Dec 6.

Published in final edited form as: J Immunol. 2010 Jun 30;185(3):1419–1428. doi: 10.4049/jimmunol.1001140

Table I.

Not all virus infections or pro-inflammatory stimuli sensitize bystander CD8 T cells¹

	Virus infection or pro-inflammatory stimulus

TCR Tg	LCMV	PV	VV	Poly(I:C)
P14	N/A	5.8±4	1.1±0.09	5.8±5
HY	0.93±0.13	0.96±0.04	0.5±0.5	0.66±0.3
OT-I	2.9±2	1.7±0.6	1.2±0.4	2.1±0.2

P14, HY, or OT-I transgenic CD8 T cells were adoptively transferred into naïve congenic recipients followed by inoculation with 5×10⁴ pfu LCMV, 1.5×10⁷ pfu PV, 1×10⁶ pfu VV, or 200 µg poly(I:C) i.p. At day 5 post infection or day 1 post poly(I:C) inoculation, splenocytes were stimulated with GP_33–41, Smcy, or SIINFEKL peptides ex vivo. Each transgenic population was gated and the ability of the transgenic T cells to produce IFN-γ in response to their cognate peptide was assessed. Numbers depict the average ratio of IFN-γ production from infected/treated mice over IFN-γ production from naïve/untreated mice stimulated with cognate peptide ± standard deviation from >30 total experiments.