Table 1.

DTH response to and rejection of minor antigen-mismatched cardiac allografts

Swine	Immunization	DTH response	Rejection
C1	–	Negative (<1 mm)	TOL
C2	–	Negative (<1 mm)	TOL
C3	–	Negative (<1 mm)	TOL
C4	–	Negative (<1 mm)	TOL
C5	Adjuvant alone	Negative (<1 mm)	TOL
P1	CM	Positive (13 mm)	ACR (ISHLT grade 3R)
P2	CM	Positive (11 mm)	ACR (ISHLT grade 3R)
P3	CM	Positive (> 10 mm)	ACR (ISHLT grade 3R)
P4	CM	Negative (8 mm)	TOL (grade 1R at day 62)

Four swine (P1–P4) were immunized twice subcutaneously with CM emulsified in CFA on day −42 and in IFA on day −21 before transplantation with a minor antigen-mismatched heart. Four control nonimmunized swine transplanted with a minor antigen-mismatched heart were also tested (C1– C4). Swine C5 was injected with adjuvant alone (day −42 with CFA, day −21 with IFA) before receiving a minor antigen-mismatched heart transplant. DTH responses >10 mm were considered positive.

CM, cardiac myosin; CFA, complete Freund’s adjuvant; IFA, incomplete Freund’s adjuvant; TOL, tolerant (i.e., allografts displaying normal function >180 d posttransplantation); ACR, acute cellular rejection; DTH, delayed type hypersensitivity; ISHLT, International Society for Heart and Lung Transplantation.