Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Biomaterials. 2011 Nov 5;33(4):1154–1161. doi: 10.1016/j.biomaterials.2011.10.033

Figure 6. Fresh and released si-NPs are delivered intercellularly and mediate gene specific silencing.

Figure 6

qRT-PCR was used to measure expression of the model gene GAPDH relative to β-actin and then normalized to no treatment controls. A) Bioactivity of freshly prepared and PUR-released si-NPs collected during the defined timeframes 0–24h, 24–48h, and 48–96h indicates that bioactivity of si-NPs released from the PUR is not significantly altered over time. Statistical significance relative to scrambled control siRNA containing si-NPs was noted at all time points (p<0.05). B) Dose response of PUR-released si-NPs demonstrated a linear relationship (R2= 0.999) between siRNA dose and silencing activity, suggesting an siRNA-dependent gene silencing effect. Minor reduction in siRNA bioactivity was apparent in PUR-released si-NPs relative to fresh si-NPs. C) Diffuse green fluorescence is noted in the cytoplasm of NIH3T3s after 4 hours of incubation with PUR-released si-NPs. This presence of FAM-labeled siRNA in the cytoplasm confirmed effective siRNA cytoplasmic delivery.