Table 1.

Viability of initial Bifidobacterium strains, acid-adapted and acid-bile-NaCl- adapted isolates in gastric and intestinal simulated fluid. Isolates were treated by subsequent incubation in acid, bile and salt environment.

	Viable count (log cfu ml−1; mean±S.D.@) during simulated gastric transit tolerance			Viable count (log cfu ml−1; mean±S.D.) during small intestinal transit tolerance
Bacterial strain	0 min¥	90 min	120 min	0 min	90 min	120 min
B. angulatum	9.18±0.04	6.63±0.12¤*	4.86±0.58 ¤*	8.35±0.13	8.09±0.15	7.55±0.22
	(9.15±0.20)#	(7.16±0.14)*	(6.63±0.14)*	ND	ND	ND
	[9.17±0.06]§	[7.23±0.06] ¤	[7.75 ±0.05] ¤	[8.45±0.16]	[8.28±0.21]	[8.10±0.25]
B. animalis	8.75±0.13	8.05±0.25 *	6.85±0.11 ¤*	9.12±0.06	9.02±0.19	8.87±0.15
	(8.78±0.11)	(8.59 ±0.73)*	(8.14 ±0.69) *	ND	ND	ND
	[8.82±0.11]	[8.71±0.15]	[8.46±0.09] ¤	[9.12±0.10]	[9.09±0.30]	[8.87±0.15]
B. bifidum	8.45±0.11	7.25±0.19 ¤	6.28±0.12 ¤*	8.87±0.10	8.56±0.25	8.11±0.10
	(8.52±0.15)	(7.57±0.18)	(7.07 ±0.08) *	ND	ND	ND
	[8.48±0.22]	[7.88±0.11] ¤	[7.47±0.39] ¤	[8.75±0.41]	[8.68±0.11]	[8.53±0.16]
B. adolescentis	9.27±0.05	4.67±0.10 ¤*	2.11±0.05 ¤*	8.95±0.11	7.41±0.05 ¤	7.05±0.08 ¤
	(9.21±0.47)	(7.25±0.31)*	(6.15 ±0.11)*	ND	ND	ND
	[9.18±0.08]	[7.86±0.08] ¤	[6.75±0.61] ¤	[8.91±0.11]	[8.13±0.21] ¤	[7.35±0.49] ¤

@ logarithmic Colony Forming Units per milliliters; mean±standard deviation

^¥Min

^#The figures quoted in parenthesis represent logarithm of survival of acid-adapted isolates.

^§The figures quoted in closed brackets represent logarithm of survival of acid-bile-NaCl-adapted isolates.

^*It shows a significant difference between survivals of acid-adapted isolates with initial strains (p<0.05).

^¤It shows a significant difference between survivals of acid-bile-NaCl-adapted isolates with initial strains (p<0.05).

ND=Not Determined