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. 2011 Nov 25;67(Pt 12):1537–1541. doi: 10.1107/S1744309111038395

Figure 2.

Figure 2

Analytical gel-filtration liquid chromatography of Vv-DHDPS. The partition coefficient (K av) is plotted as a function of log10(molecular weight). Experiments were performed on a 10 × 300 mm Superose 12 column using ovalbumin (44 kDa), conalbumin (75 kDa), aldolase (158 kDa) and catalase (240 kDa) as globular protein standards (open circles) and employing blue dextran (2000 kDa) and riboflavin (376 Da) to determine the void and total volume of the column. Vv-­DHDPS (red diamond) elutes at approximately 131 kDa, suggesting that the enzyme adopts either a trimeric or tetrameric quaternary structure (the M r of Vv-­DHDPS is 37 876).