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Criteria for breakapart fluorescence in situ hybridization positivity for detection of ALK-rearranged non-small cell lung cancer
| Characteristics | Criteria for positivity |
|---|---|
| Number of cells counted | At least 50 cells |
| Percentage of positive signals | >15% |
| Distance between 5′ (green) and 3′ (red) ALK signals | Greater than two signal diameter separation |
| Presence of single 3′ ALK (red) signal only | Yes |
| Presence of single 5′ ALK (green) signal only | No |
