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. Author manuscript; available in PMC: 2012 Dec 8.
Published in final edited form as: Cancer Lett. 2011 Jul 22;311(2):177–186. doi: 10.1016/j.canlet.2011.07.015

Fig.2.

Fig.2

Dosage and kinetic effects of HDAC inhibitors on cPAcP protein expression in LNCaP C-81 cells. LNCaP C-81 cells that were seeded at a density of 5×105 cells/T25 for 2 days in regular medium were treated with different concentrations of (A) NaB or (B) VPA for 48 hr, or treated with (C) 1 mM NaB or (D) 1 mM VPA for different time periods. The total protein was harvested and subjected to western blot analyses of cPAcP protein expression. (D) The acetylation and the methylation levels of H3 and H4 proteins were also analyzed in VPA-treated C-81 cells for different time periods. β-Actin was analyzed and used as a loading control in each experiment. Similar results were obtained from five sets of independent experiments.