Fig. 5.

Effects of HDAC inhibitors on cell cycle distribution, apoptosis and cell cycle protein expression in LNCaP C-81 cells. LNCaP C-81 cells were plated in regular medium for 48 hr and treated with 1 mM NaB, 1 mM VPA or 300 nM TSA for 48 hr. All cells were harvested and subjected to cell cycle analyses using flow cytometry and western blot analyses of indicated proteins. (A) Cell cycle analyses. The data shown is the mean of three sets of independent experiments. (B) Western blot analyses. The total cellular lysate proteins were analyzed for cPAcP protein, ErbB-2 phosphorylation, histone H3/H4 acetylation and methylation, AR, cPSA, cyclin B1 and D1, PCNA and p21 protein levels. β-Actin was analyzed as a loading control in each experiment. The data shown is a representative of three sets of independent experiments.