Table 2. Primers used in gene-specific qRT-PCR of flowering genes.
Gene name | Primer sequence (5′-3′) |
GhCCA1 | F:TCATTGTAGGGATGCGGCTGTT |
R:CGAGTTGCTGGTGGATGGGTT | |
GhCRY1 | F:ATGCCCAGATCATTTATCCATAAG |
R:AATAATGGGAATTGGCTCTGG | |
GhFRI | F:TCTCCGACTGCTTTATCAGGTTCTGC |
R:GATCCTGGCGAGTTGACCGAGTTA | |
GhVIN3 | F:GAGATGCTGGATCAGAAATGAAGA |
R:GGAGAACGACAAGACGAGGAAT | |
GhFLD | F:GCCGAAGTCAATTTCTTCCTCA |
R:CCAATGTTCCATACTCTGACCCTAA | |
GhSOC1 | F:AACGCCTTCTTTAGCAAACCAT |
R:ACCCTACAAGCAGGCAAGTGA | |
GhUFO | F:GCCACTGCTGCCAAGGTAAG |
R:CGGACAAGGGACTGCTGTTT | |
GhGI | F:AGAGGGACCACGGAAACCA |
R:CAGCACATCAGTCCTTCGCAAT | |
GhCAL | F:TCTCCACGAAAGTTCCTCAA |
R:GGTTCTGAATCACAGGCAAA | |
GhSVP | F:GAACTTCTTGATTGACCTGCTCTAA |
R:GTGCTGGACTGCATGAAGGATAT | |
GhSPY | F:TCCGTCACAGACAGGTGATTTAG |
R:CAATGTCGGTGTCAGTCTTCTCA | |
GhAG | F:CCAGCATGTGCCTGTTTGTATT |
R:ATTGTCTTCTCCAACCGTGGTC | |
GhSEP1 | F:TCCGCTCCACCAAGACCC |
R:GACAAAGCCCTGTTAGTTTCCAT | |
GhSEP2 | F:AACCAACCCATCAGCCTCAG |
R:GGTAGCCATCCCGTCATGTAAT | |
GhSEP3 | F:AATGAAGTTGGATGGAAGTGGTC |
R:AGGTGGTGGATGGTTGTAT | |
18S | F:AACCAAACATCTCACGACAC |
R:GCAAGACCGAAACTCAAAG |