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. 2011 Dec 6;6(12):e28557. doi: 10.1371/journal.pone.0028557

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Garzón et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A. Recombinant RGSZ2 and its RH region were incubated with SUMO1- (lane 3), SUMO2- (lane 4) and SUMO3-agarose (lane 5). SUMO-agarose conjugates captured RGSZ2 and its RH domain. Lane 1, RGSZ2/RH added. Lane 2, RGSZ2/RH in presence of agarose without SUMO. B. The Gαi2 subunit shows no binding to SUMO1/2/3-agarose.