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Sub-stock solutions were diluted with MeOH from the stock solutions and used to prepare calibration standards and QC samples as follows.
| Target concentration (µg/mL) |
Initial concentration (µg/mL) |
Solution (mL) |
Final volume (mL) |
Final concentration (µg/mL) |
|
|---|---|---|---|---|---|
| Substock 1 | 100 | 1000 | 0.5 | 5 | 100 |
| Substock 2 | 10 | 100 | 0.5 | 5 | 10 |
| Substock 3 | 1 | 10 | 0.5 | 5 | 1 |
