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The 1H-15N TROSY spectra of free hUNG and the effect of the Gd(HP-DO3A) paramagnetic cosolute. (a) The spectra were obtained with isotropically 2H, 15N-enriched hUNG samples with no Gd(HP-DO3A) (black) or [Gd(HP-DO3A)] = 2.5 mM (red). (b) Representative 1D slices (f1 = 123.8 ppm) of the spectra in (a).
