Figure 4. Morphological analysis reveals atypical morphology of activated microglia isolated from TNF-null mice.
Primary microglia isolated from wild type or TNF-null postnatal day 3–5 (P3–5) pups were treated 24-hours post-plating with 10 ng/mL or 1 µg/mL lipopolysaccharide (LPS). Cells were fixed and stained with an anti-CD45 antibody to assess microglia morphology. Scale bar = 50 µm.