PCR-DGGE profile of bacterial 16S rDNA gene fragments of BRONJ tissue samples with and without antibiotics. Marker I & II: DGGE reference markers correspond to 16S rRNA gene fragments from quoted specific bacterial species [Marker I: 1. Fusobacterium nucleatum subsp. vincenti (ATCC 49256); 2. Fusobacterium nucleatum subsp. nucleatum (ATCC 25586); 3. Streptococcus sanguinis (ATCC 10556); 4. Streptococcus oralis (ATCC 35037); 5. Streptococcus salivarus (ATCC 7073); 6. Streptococcus mutans (UA 159); 7. Lactobacillus paracasei (ATCC 25598); 8. Porphyromonas gingivalis (ATCC 33277); 9. Actinomyces odontolyticus (ATCC 17929); 10. Actinomyces naeslundii (ATCC 12104), Marker II: F. nucleatum subsp. vincenti (ATCC 49256); 2. F. nucleatum subsp. nucleatum (ATCC 25586); 3. Bacteroides forsythus (ATCC 43037) (T.f.); 4. S. sanguinis (ATCC 10556); 5. S. oralis (ATCC 35037); 6. Veillonella parvula (ATCC 17745); 7. Prevotella intermedia (ATCC 25611); 8. Aggregatibacter actinomycemcomitans (ATCC 43717); 9. P. gingivalis (ATCC 33277); 10. A. odontolyticus (ATCC 17929); 11. A. naeslundii (ATCC 12104)]
A - BRONJ tissue samples with antibiotics; NA - BRONJ tissue samples without antibiotics
* - light bands not visible in photograph