. Author manuscript; available in PMC: 2012 Dec 1.

Published in final edited form as: Curr Opin Struct Biol. 2011 Oct 23;21(6):728–734. doi: 10.1016/j.sbi.2011.09.005

Table 1.

Crystal Structures of Rtt109-Vps75

PDB Code	Purificatino Procedure	Tagged Protein	Res. (Å)	Space Group	Vps75: Rtt109	Other	Interface¹	Ref
n/a	1. Ni-NTA AC 2. Cation EC 3. SEC	Rtt109	4.3	P2₁	2:1	Low resolution prevented refinement	II^2,3	[20]
3Q66	1. GST AC 2. SEC	Rtt109	2.7	P6₁22	2:1	Protein buffer had acetyl-CoA; no acetyl-CoA in PDBs	I, II, III	Su et al. [27]
3Q68	1. GST AC 2. SEC	Rtt109	2.7	P2₁2₁2₁	2:1	Protein buffer had acetyl-CoA; no acetyl-CoA in PDBs	I, II, III	Su et al. [27]
3Q33	1. Ni-NTA AC 2. Anion EC 3. SEC	Vps75	2.8	P2₁2₁2₁	2:2	Crystal soaked with CoA and an Ac-peptide	I, II³	Tang et al. [22]
3Q35	1. Ni-NTA AC 2. Anion EC 3. SEC	Vps75	3.3	P2₁2₁2₁	2:2	-	I, II³	Tang et al. [22]

See Figure 2.

nterface I not observed as limited resolution prevented building beyond the Rtt109 alone and Vps75 alone molecular replacement search models [20].

Interface III not observed as a truncated Vps75 construct was used [22].

Abbreviations: PDB – Protein Databank, AC - Affinity chromatography, EC - Exchange Chromatography, SEC – Size Exclusion Chromatography, Res. – Resolution, Ref - Reference