Fig. 3.

Intravenous immunoglobulin (IVIg)-treated Jurkat T cells are responsive to phytohaemagglutinin (PHA) activation. Jurkat T cells were incubated for 4 h in the presence of IVIg (10 mg/ml) (step 1), followed by three washes and PHA activation (0·5 µg/ml) in the presence or absence of 10 mg/ml of IVIg (step 2). Cells were incubated to 24 h prior to interleukin (IL)-2 determination by enzyme-linked immunosorbent assay (ELISA). Results [± standard deviation (s.d.)] are representative of five independent experiments. n.s.: not significant, **P < 0·01; ***P < 0·001 (Kruskal–Wallis with Dunn's post-test).