Table 2.

Oligonucleotide primers and PCR programs used for amplifying the genes encoding 23S rRNA along with various other staphylococcal proteins including toxins and adhesive molecules

^*1: 37 cycles of 94℃ for 40 sec, 64℃ for 60 sec, and 72℃ for 75 sec; 2: 37 cycles of 94℃ for 60 sec, 55℃ for 30 sec, and 72℃ for 30 sec; 3: 30 cycles of 94℃ for 60 sec, 58℃ for 60 sec, and 72℃ for 60 sec; 4: 35 cycles of 94℃ for 60 sec, 57℃ for 60 sec, and 72℃ for 60 sec; 5: 30 cycles of 94℃ for 60 sec, 60℃ for 60 sec, and 72℃ for 60 sec; 6: 30 cycles of 94℃ for 120 sec, 55℃ for 120 sec, and 72℃ for 60 sec; 7: 30 cycles of 94℃ for 60 sec, 62℃ for 60 sec, and 72℃ for 60 sec; 8: 30 cycles of 94℃ for 30 sec, 50℃ for 30 sec, and 72℃ for 60 sec; 9: 20 cycles of 94℃ for 10 sec, 53℃ for 10 sec, and 72℃ for 30 sec; 10: 20 cycles of 94℃ for 10 sec, 62℃ for 10 sec, and 72℃ for 30 sec; 11: 20 cycles of 94℃ for 15 sec, 57℃ for 15 sec, and 72℃ for 30 sec; 12: 20 cycles of 94℃ for 15 sec, 52℃ for 15 sec, and 72℃ for 30 sec.