Table 1.

Screening of Fluorescent Substrate Cleavage Activity by MMPs and ADAMs

	Flsub10c	Flsub21b	Flsub13b	Flsub63a	Flsub8b	Flsub11a
ADAM8	3.3 × 104	1.0 × 105	5.3 × 104	2.5 × 103	1.7 × 103	2.4 × 103
ADAM9	1.1 × 105	8.3 × 103	1.6 × 103	2.2 × 104	4.5 × 103	2.3 × 102
ADAM10	3.6 × 103	6.2 × 103	2.7 × 102	1.4 × 104	1.9 × 102	<1 × 101
ADAM12	3.8 × 104	2.8 × 105	4.0 × 101	NAd	3.0 × 103	NAd
ADAM17	9.6 × 105	4.3 × 105	NDe	1.1 × 103	3.8 × 104	3.7 × 103
MMP-1	5.1 × 104	2.8 × 104	NDe	3.2 × 103	7.6 × 104	4.5 × 104
MMP-2	1.7 × 105	3.2 × 105	2.4 × 103	1.3 × 106	2.9 × 104	4.8 × 105
MMP-3	3.9 × 104	4.0 × 103	NDe	2.2 × 104	5.2 × 101	1.6 × 103
MMP-8	2.6 × 104	1.4 × 105	NDe	3.4 × 105	2.4 × 104	3.6 × 104
MMP-9	6.0 × 105	2.2 × 105	NDe	1.4 × 105	8.5 × 105	1.4 × 106
MMP-13	1.7 × 106	4.6 × 105	NDe	1.6 × 106	2.1 × 106	3.3 × 106

^aSpecificity values at 10 µM substrate concentration were measured by back calculation of enzyme concentrations after determination of k_cat/K_m for known substrates. Enzyme concentrations were determined previously by active site titration as described in Rasmussen et. al. [34]

^bValues were taken from Moss et. al. [35]

^cValues were taken from Moss et. al. [42]

^dNot attempted

^eNo activity detected