Fig. 4.

Collagen V localization in large vessels in obese subjects. Adipose tissue sections from lean (A–C) and obese (D–F) subjects were immunoreacted with antibodies against ASMA (B and E, green) and collagen V (C and F, red) to localize collagen V to blood vessels in merged images (A and D). Vessels from lean subjects exhibited very little collagen V accumulation. Vessels from obese subjects contained significantly more collagen V. In most vessels in obese subjects, collagen V could be seen to extend from the endothelial basement membrane to the outer edges of the vessel. Magnification, ×200; bar, 125 μm.