Plasma kisspeptin IR and serum reproductive hormone levels after sc bolus injection of kisspeptin-10 to healthy female volunteers in the follicular phase of the menstrual cycle. A–D, Time profiles for plasma kisspeptin IR (A) and changes in serum LH (B), FSH (C), and estradiol (D) during 4 h after sc bolus injection of kisspeptin-10 to healthy female volunteers in the follicular phase of the menstrual cycle (n = 4–5 per group). For 32 vs. 2 nmol/kg: *, P < 0.05; ***, P < 0.001. For 16 vs. 2 nmol/kg: ƒƒƒ, P < 0.001. For 8 vs. 2 nmol/kg: ψψψ, P < 0.001. For 4 vs. 2 nmol/kg: λ, P < 0.05. E–H, AUC for plasma kisspeptin IR (E) and changes in serum LH (F), FSH (G), and testosterone (H) during 4 h after sc bolus injection of kisspeptin-10 to healthy female volunteers in the follicular phase of the menstrual cycle (n = 4–5 per group). *, P < 0.05; **, P < 0.01; ***, P < 0.001. E2, Estradiol. Data are shown as mean ± sem.