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. 2011 Dec;79(12):4947–4956. doi: 10.1128/IAI.05640-11

Fig. 1.

Fig. 1.

Proinflammatory cytokine induction in mouse BMDMs by E. chaffeensis is MyD88 dependent but TRIF independent. (A) Dose response of IL-1β, CXCL2, and TNF-α induction in mouse BMDMs to E. chaffeensis Wakulla was determined by quantitative RT-PCR. Bacteria were added at different IFU/ml to each well, and the plates were incubated at 37°C for 2 h. The transcript amount of each gene is represented as expression relative to untreated BMDMs. All data are normalized to mouse GAPDH mRNA. Mock, extract of uninfected DH82 cells corresponding to 20 IFU of Ehrlichia/cell. (B) IL-1β, CXCL2, and TNF-α induction in BMDMs from MyD88, TRIF, or MyD88/TRIF double-knockout mice (KO). Bacteria derived from 5 × 105 infected cells were added/well and incubated at 37°C for 2 h. C57BL/6 WT, wild-type control. −, PBS. Data shown are means and standard deviations from triplicate assays. **, significantly different from each other (ANOVA, P < 0.01). NS, not significantly different (ANOVA, P > 0.05). Data are representative of results of at least three independent experiments.