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HeLa cell invasion by S. dysenteriae vaccine strains. Bacteria (strains 1617, CVD 1254, CVD 1255, etc.) were incubated with HeLa cell monolayers at an MOI of 100 for 90 min and washed with phosphate-buffered saline (PBS) containing gentamicin. The cells were lysed to enumerate intracellular bacteria at 0 h and 4 h postinfection.
