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. 2011 Dec;79(12):4791–4801. doi: 10.1128/IAI.05808-11

Fig. 1.

Fig. 1.

Autoantibody secretion from purified murine splenic B cells as well as their sorted B-1 or B-2 cells stimulated with purified H. pylori urease. (A) Purified B cells (1 × 106) were cultured with 10 μg/ml purified H. pylori urease from either the SS-1 or NCTC 11637 isolate for 7 days, and the supernatants were harvested to measure their anti-ssDNA autoantibody production by ELISA. Data are means ± SDs (n = 6). (B) Either 2 × 105 purified B cells or the same number of their sorted B-1a or B-2 cells were cultured with 10 μg/ml purified H. pylori urease (filled columns) or PBS (open columns) for 7 days in CM, and the supernatants were harvested to measure either their anti-ssDNA (top) or anti-PC (bottom) autoantibody production. Data are means ± SDs (n = 6). O.D., optical density. (C) Purified peritoneal cavity-derived B-1a (perC B-1a) cells (1 × 105) were cultured with 10 μg/ml purified H. pylori urease for 7 days, and the supernatants were harvested to measure their anti-ssDNA autoantibody production. Statistical significance was determined by the Student t test, and the values are given in each graph.