Fig. 4.
Secretion of autoantibodies from purified B-1a cells stimulated with fixed plate-coated H. pylori. Purified murine B-1a cells (2 × 105) were cultured with either urease-positive H. pylori (SS-1 and NCTC 11637) or urease-negative H. pylori (HPP1801) fixed by 2% PFA for 10 min and cultured for an additional 7 days, and the supernatants were harvested to measure the production of autoantibodies by ELISA. Results are expressed as means ± SDs (n = 5). Statistical significance was determined by the Student t test, and the P values are given in each graph.