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. 2011 Dec;79(12):4791–4801. doi: 10.1128/IAI.05808-11

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Copyright © 2011, American Society for Microbiology. All Rights Reserved.

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Fig. 9. — Detection of H. pylori LPS on/in mutant HPP1801 by immunohistological staining and Western blotting. We tried to identify H. pylori LPS (Le^x and Le^y antigens) in/on the urease-negative HPP1801 mutant that did not stimulate B-1a cells by anti-Le^x (clone P12) or anti-Le^y (clone F3) murine IgM MAbs. (A) Both Le^x and Le^y were apparently expressed on the surface of mutant HPP1801 as well as isolates NCTC 11637 and SS-1 by light microscopy. (B) Le^x and Le^y antigens were seen in the purified LPS obtained from HPP1801 as well as NCTC 11637 and SS-1 by Western blotting.