Fig. 1.

Distinct gene expression profiles obtained from mouse J774 macrophages stimulated with live B. burgdorferi (Bb) spirochetes alone or in combination with IL-10 at 4 h (A) and 24 h (B) and common genes between 4 h and 24 h (C) poststimulation. Macrophages (3 × 10⁶/ml) were incubated with live B. burgdorferi spirochetes at an MOI of 10 in the presence or absence of 10 ng/ml of mouse recombinant IL-10. The control culture consisted of cells incubated with medium alone (unstimulated). Microarray analysis was conducted on the cDNA produced from RNA extracted from macrophages at 4 h and 24 h poststimulation. Data are reported as fold change induction relative to the values obtained from unstimulated cells. Genes whose expression changed by at least 2-fold or more for upregulated genes and −2-fold or less for downregulated genes (with a corrected one-way analysis of variance, P < 0.05) compared with unstimulated cells were considered to be differentially expressed in a statistically significant manner. The mean fold induction of the expression of each sample was expressed as a ratio of intensities of stimulated to unstimulated cells in two parallel experiments.