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. 2011 Dec;79(12):4876–4892. doi: 10.1128/IAI.05451-11

Table 2.

Selected upregulated gene transcripts in macrophages 4 h after exposure to live B. burgdorferi which were potentiated in the presence of added exogenous IL-10a

Functional group and description Gene no. Annotation Fold change in expression by culture condition(s)
 Fold change ratio (live Bb culture/live Bb + IL-10 culture)
Live Bbb Live Bb + IL-10
Cytokines
    Interleukin 1 beta NM_008361 Il1b 146.37 180.93 0.81
    Interleukin 10 NM_010548 Il10 3.81 4.74 0.8
    Chemokine (C-C motif) ligand 9 NM_011338 Ccl9 4.18 5.62 0.74
    Chemokine (C-C motif) ligand 4 NM_013652 Ccl4 11.32 16.23 0.7
    Interleukin 1 receptor antagonist NM_031167 Il1rn 3.91 6.79 0.57
Enzymes
    RAB12, member RAS oncogene family NM_024448 Rab12 2.26 2.75 0.82
    Superoxide dismutase 2, mitochondrial NM_013671 Sod2 6.41 8.33 0.77
    Diacylglycerol O-acyltransferase 2 NM_026384 Dgat2 3.15 4.47 0.71
    CTAGE family, member 5 AK164018 Mgea6 2.08 3.05 0.68
    Carbonic anhydrase 13 NM_024495 Car13 5.5 8.82 0.62
    UDP-glucose ceramide glucosyltransferase NM_011673 Ugcg 3.37 5.5 0.61
    Sphingomyelin synthase 1 NM_144792 Tmem23 3.91 6.49 0.6
    Prostaglandin-endoperoxide synthase 2 NM_011198 Ptgs2 15.95 27.45 0.58
G-protein coupled receptor
    Adenosine A2b receptor NM_007413 Adora2b 2.98 6.85 0.44
Ion channel
    Mucolipin 2 NM_026656 Mcoln2 2.96 4.05 0.73
Kinases
    Proviral integration site 3 NM_145478 Pim3 2.85 3.6 0.79
    Phosphoinositide-3-kinase, regulatory subunit 5, p101 NM_177320 Pik3r5 3.1 4.03 0.77
    Inhibitor of κB kinase epsilon NM_019777 Ikbke 2.93 3.95 0.74
    Proviral integration site 1 NM_008842 Pim1 2.18 2.99 0.73
    Mitogen-activated protein kinase kinase kinase kinase 4 AK020498 9430080K19Rik 2.4 3.49 0.69
    Hemopoietic cell kinase NM_010407 Hck 2.6 4.19 0.62
    Unknown NM_010884 Ndrg1 2.68 5.9 0.46
Peptidases
    Caspase 1 NM_009807 Casp1 2.58 3.48 0.74
    Matrix metallopeptidase 13 NM_008607 Mmp13 8.11 11.74 0.69
    A disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 NM_009621 Adamts1 2.73 6.27 0.44
Phosphatase
    Dual specificity phosphatase 1 NM_013642 Dusp1 3.51 5.71 0.61
Transcription regulators
    B-cell leukemia/lymphoma 3 NM_033601 Bcl3 4.52 5.47 0.83
    E2F transcription factor 5 X86925 E2f5 2.54 3.49 0.73
    Hypoxia inducible factor 1, alpha subunit NM_010431 Hif1a 3.44 4.78 0.72
    Kruppel-like factor 7 (ubiquitous) NM_033563 Klf7 2.22 3.09 0.72
    Activating transcription factor 3 NM_007498 Atf3 6.87 9.65 0.71
    Microphthalmia-associated transcription factor NM_008601 Mitf 2.86 4.4 0.65
    Nuclear factor, interleukin 3, regulated NM_017373 Nfil3 2.81 4.78 0.59
    MAX dimerization protein 1 AK137548 Mxd1 2.99 5.53 0.54
    Signal transducer and activator of transcription 3 NM_213659 Stat3 2.91 5.89 0.49
    CCR4 carbon catabolite repression 4-like (S. cerevisiae) NM_009834 Ccrn4l 4.74 10.29 0.46
Transmembrane receptors
    CD40 antigen NM_170701 Cd40 3.26 4.18 0.78
    Fc receptor, IgG, low-affinity IIb NM_010187 Fcgr2b 7.83 15.52 0.5
    Fc receptor, IgG, low-affinity III NM_010188 Fcgr3 3.3 6.63 0.5
    Tumor necrosis factor receptor superfamily, member 9 NM_011612 Tnfrsf9 3.09 5.42 0.57
Transporters
    Solute carrier family 16 (monocarboxylic acid transporters), member 1 NM_009196 Slc16a1 2.22 3.01 0.74
    Synaptotagmin X NM_018803 Syt10 2.47 3.89 0.64
    Serum amyloid A 1 NM_009117 Saa1 20.57 64.77 0.32
    Serum amyloid A 3 NM_011315 Saa3 34 66.07 0.51
Other
    TNFAIP3 interacting protein 3 NM_001001495 TNIP3 4.19 14.90 0.28
    Tumor necrosis factor, alpha-induced protein 3 NM_009397 Tnfaip3 19.96 58.62 0.34
    SAM domain, SH3 domain and nuclear localization signals, 1 NM_023380 Samsn1 3.04 6.97 0.44
    RIKEN cDNA 4933426M11 gene NM_178682 4933426M11Rik 2.45 2.97 0.82
    Unknown AK031731 Nfe2l2 2.21 2.71 0.82
    Zinc finger, AN1-type domain 5 NM_009551 Zfand5 2.15 2.67 0.81
    Phosphodiesterase 4B, cAMP specific NM_019840/AK171700 Pde4b 6.83 9.07 0.75
    RIKEN cDNA 1810022K09 gene BC045157 1810022K09Rik 2.64 3.65 0.72
    Unknown AT_ssM_RR_3 AT_ssM_RR_3 2.46 3.47 0.71
    RIKEN cDNA 1810029B16 gene NM_025465 1810029B16Rik 3.35 4.75 0.71
    Mesoderm development candidate 1 NM_030705 Mesdc1 2.15 3.09 0.7
    Pleckstrin NM_019549 Plek 2.64 3.81 0.69
    Ral guanine nucleotide dissociation stimulator, -like 1 NM_016846 Rgl1 3.09 4.59 0.67
    Immediate-early response 3 NM_133662 Ier3 3.72 5.71 0.65
    CDNA sequence BC031781 NM_145943 BC031781 2.43 3.82 0.64
    Ring finger protein 149 NM_001033135 Rnf149 2.68 4.3 0.62
    RIKEN cDNA E130014J05 gene NM_001040400 E130014J05Rik 2.33 3.75 0.62
    RIKEN cDNA 5730508B09 gene AK162420/NM_027482 5730508B09Rik 4.07 9.32 0.44
    Unknown AK035396 1200016E24Rik 3.71 8.91 0.42
    Mitochondrial ribosomal protein L52 AK081551 Mrpl52 3.20 6.20 0.52
    Activity regulated cytoskeletal-associated protein NM_018790 Arc 3.83 6.67 0.57
a

A corrected one-way analysis of variance was used to analyze the microarray data. Genes whose expression levels were upregulated by at least 2-fold or more (P < 0.05) compared to unstimulated cells were considered to be differentially expressed in a statistically significant manner. The underlined genes are common to both the 4- and 24-h time points.

b

Bb, B. burgdorferi spirochetes.