Table 4.

Selected upregulated gene transcripts in macrophages 24 h after exposure to live Borrelia burgdorferi which were potentiated in the presence of added exogenous IL-10^a

Functional group and description	Gene no.	Annotation	Fold change in expression by culture condition(s)		Fold change ratio (live Bb culture/live Bb + IL-10 culture)
			Live Bbb	Live Bb + IL-10
Cytokines
Chemokine (C-C motif) ligand 4	NM_013652	Ccl4	4.52	5.44	0.83
Chemokine (C-C motif) ligand 2	NM_011333	Ccl2	13.33	16.69	0.8
Chemokine (C-C motif) ligand 9	NM_011338	Ccl9	3.03	3.92	0.77
Interleukin 1 beta	NM_008361	Il1b	52.97	83.67	0.63
Chemokine (C-C motif) ligand 12	NM_011331	Ccl12	2.59	5.99	0.43
Chemokine (C-C motif) ligand 7	NM_013654	Ccl7	8.24	34.01	0.24
Enzymes
Prolyl 4-hydroxylase, beta polypeptide	NM_011032	P4hb	2.7	3.23	0.83
Ras homolog gene family, member Q	NM_145491	Rhoq	2.31	2.82	0.82
Phosphodiesterase 4B, cAMP specific	NM_019840	Pde4b	3.94	5.02	0.79
Glutaredoxin	NM_053108	Glrx	2.95	3.98	0.74
DNA segment, Chr 1, Brigham and Women's Genetics 1363 expressed	NM_001001566	D1Bwg1363e	2.39	3.31	0.72
Bone marrow stromal cell antigen 1	NM_009763	BstI	2.69	6.54	0.41
G-protein coupled receptor
Formyl peptide receptor 1	NM_013521	Fpr1	2.84	5.15	0.55
Peptidases
HtrA serine peptidase 1	NM_019564	Htra1	5.1	9.65	0.53
Matrix metallopeptidase 13	NM_008607	Mmp13	2.62	12.5	0.21
Phosphatases
Dual specificity phosphatase 2	NM_010090	Dusp2	2.4	5.85	0.41
Protein tyrosine phosphatase, receptor type, J	NM_008982	Ptprj	2.11	7.22	0.29
Transcription regulators
Zinc finger protein 36	NM_011756	Zfp36	2.25	3.45	0.65
CCR4 carbon catabolite repression 4-like (S. cerevisiae)	NM_009834	Ccrn4l	2.95	4.66	0.63
B-cell leukemia/lymphoma 3	NM_033601	Bcl3	3.9	7.12	0.55
FBJ osteosarcoma oncogene	NM_010234	Fos	2.53	4.77	0.53
Transmembrane receptors
C-type lectin domain family 4, member a2	NM_011999	Clec4a2	3.29	5.28	0.62
Colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage)	AK154286	Csf2rb1	2.29	4.05	0.57
Fc receptor, IgG, high affinity I	BC025535	Fcgr1	2.25	5.82	0.39
Interleukin 4 receptor, alpha	NM_001008700	Il4ra	2.91	11.12	0.26
Transporters
Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2	NM_172980	Slc28a2	3.53	5.44	0.65
Lipocalin 2	NM_008491	Lcn2	12.13	20.55	0.59
Other
Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha	NM_010907	Nfkbia	5.76	6.9	0.83
Mitochondrial ribosomal protein L52	NM_026851	Mrpl52	3.35	4.04	0.83
DNA segment, Chr 3, University of California at Los Angeles 1	NM_030685	D3Ucla1	2.6	3.12	0.83
Ring finger protein 149	NM_001033135	Rnf149	3.01	3.72	0.81
Transmembrane protein 176A	NM_025326	0610011I04Rik	2.75	3.4	0.81
RIKEN cDNA 1200002N14 gene	NM_027878	1200002N14Rik	2.67	3.3	0.81
RIKEN cDNA 1190003J15 gene	AK004470/BC051545	1190003J15Rik	93.75	123.62	0.76
Syndecan 4	NM_011521	Sdc4	2.23	3.02	0.74
Serum amyloid A 1	NM_009117	Saa1	90.62	123.61	0.73
Interferon induced transmembrane protein 2	NM_030694	Ifitm2	2.19	2.98	0.73
Rho guanine nucleotide exchange factor (GEF) 3	NM_027871	Arhgef3	2.84	4	0.71
Myristoylated alanine rich protein kinase C substrate	NM_008538	Marcks	2.72	3.89	0.7
FGF receptor activating protein 1	NM_145583/AK152420	Frag1	2.33	3.35	0.7
RIKEN cDNA 5730508B09 gene	AK162420/NM_027482	5730508B09Rik	2.71	4.07	0.67
Ral guanine nucleotide dissociation stimulator, -like 1	NM_016846	Rgl1	5.28	7.92	0.67
Leucine rich repeat containing 25	NM_153074	Lrrc25	2.92	4.6	0.64
Transmembrane protein 176B	NM_023056	1810009M01Rik	3.26	5.18	0.63
Unknown	ENSMUST00000094405	ENSMUST00000094405	4.02	6.52	0.62
Transformed mouse 3T3 cell double minute 4	NM_008575	Mdm4	3.24	5.49	0.59
C-type lectin domain family 4, member a3	AK156040	Clec4a3	2.92	6.68	0.44
Predicted gene, OTTMUSG00000000971	BC089618	BC089618	2.92	7.86	0.37
CD244 natural killer cell receptor 2B4	NM_018729	Cd244	8.25	25.88	0.32
C-type lectin domain family 4, member b1	NM_027218	Clec4b1	3.06	9.47	0.32

^aA corrected one-way analysis of variance was used to analyze the microarray data. Genes whose expression levels were upregulated at least 2-fold (P < 0.05) compared to unstimulated cells were considered to be differentially expressed in a statistically significant manner. The underlined genes are common to both the 4- and 24-h time points.

^bBb, B. burgdorferi spirochetes.