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. 2011 Dec;79(12):4876–4892. doi: 10.1128/IAI.05451-11

Table 4.

Selected upregulated gene transcripts in macrophages 24 h after exposure to live Borrelia burgdorferi which were potentiated in the presence of added exogenous IL-10a

Functional group and description Gene no. Annotation Fold change in expression by culture condition(s)
 Fold change ratio (live Bb culture/live Bb + IL-10 culture)
Live Bbb Live Bb + IL-10
Cytokines
    Chemokine (C-C motif) ligand 4 NM_013652 Ccl4 4.52 5.44 0.83
    Chemokine (C-C motif) ligand 2 NM_011333 Ccl2 13.33 16.69 0.8
    Chemokine (C-C motif) ligand 9 NM_011338 Ccl9 3.03 3.92 0.77
    Interleukin 1 beta NM_008361 Il1b 52.97 83.67 0.63
    Chemokine (C-C motif) ligand 12 NM_011331 Ccl12 2.59 5.99 0.43
    Chemokine (C-C motif) ligand 7 NM_013654 Ccl7 8.24 34.01 0.24
Enzymes
    Prolyl 4-hydroxylase, beta polypeptide NM_011032 P4hb 2.7 3.23 0.83
    Ras homolog gene family, member Q NM_145491 Rhoq 2.31 2.82 0.82
    Phosphodiesterase 4B, cAMP specific NM_019840 Pde4b 3.94 5.02 0.79
    Glutaredoxin NM_053108 Glrx 2.95 3.98 0.74
    DNA segment, Chr 1, Brigham and Women's Genetics 1363 expressed NM_001001566 D1Bwg1363e 2.39 3.31 0.72
    Bone marrow stromal cell antigen 1 NM_009763 BstI 2.69 6.54 0.41
G-protein coupled receptor
    Formyl peptide receptor 1 NM_013521 Fpr1 2.84 5.15 0.55
Peptidases
    HtrA serine peptidase 1 NM_019564 Htra1 5.1 9.65 0.53
    Matrix metallopeptidase 13 NM_008607 Mmp13 2.62 12.5 0.21
Phosphatases
    Dual specificity phosphatase 2 NM_010090 Dusp2 2.4 5.85 0.41
    Protein tyrosine phosphatase, receptor type, J NM_008982 Ptprj 2.11 7.22 0.29
Transcription regulators
    Zinc finger protein 36 NM_011756 Zfp36 2.25 3.45 0.65
    CCR4 carbon catabolite repression 4-like (S. cerevisiae) NM_009834 Ccrn4l 2.95 4.66 0.63
    B-cell leukemia/lymphoma 3 NM_033601 Bcl3 3.9 7.12 0.55
    FBJ osteosarcoma oncogene NM_010234 Fos 2.53 4.77 0.53
Transmembrane receptors
    C-type lectin domain family 4, member a2 NM_011999 Clec4a2 3.29 5.28 0.62
    Colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) AK154286 Csf2rb1 2.29 4.05 0.57
    Fc receptor, IgG, high affinity I BC025535 Fcgr1 2.25 5.82 0.39
    Interleukin 4 receptor, alpha NM_001008700 Il4ra 2.91 11.12 0.26
Transporters
    Solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 NM_172980 Slc28a2 3.53 5.44 0.65
    Lipocalin 2 NM_008491 Lcn2 12.13 20.55 0.59
Other
    Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha NM_010907 Nfkbia 5.76 6.9 0.83
    Mitochondrial ribosomal protein L52 NM_026851 Mrpl52 3.35 4.04 0.83
    DNA segment, Chr 3, University of California at Los Angeles 1 NM_030685 D3Ucla1 2.6 3.12 0.83
    Ring finger protein 149 NM_001033135 Rnf149 3.01 3.72 0.81
    Transmembrane protein 176A NM_025326 0610011I04Rik 2.75 3.4 0.81
    RIKEN cDNA 1200002N14 gene NM_027878 1200002N14Rik 2.67 3.3 0.81
    RIKEN cDNA 1190003J15 gene AK004470/BC051545 1190003J15Rik 93.75 123.62 0.76
    Syndecan 4 NM_011521 Sdc4 2.23 3.02 0.74
    Serum amyloid A 1 NM_009117 Saa1 90.62 123.61 0.73
    Interferon induced transmembrane protein 2 NM_030694 Ifitm2 2.19 2.98 0.73
    Rho guanine nucleotide exchange factor (GEF) 3 NM_027871 Arhgef3 2.84 4 0.71
    Myristoylated alanine rich protein kinase C substrate NM_008538 Marcks 2.72 3.89 0.7
    FGF receptor activating protein 1 NM_145583/AK152420 Frag1 2.33 3.35 0.7
    RIKEN cDNA 5730508B09 gene AK162420/NM_027482 5730508B09Rik 2.71 4.07 0.67
    Ral guanine nucleotide dissociation stimulator, -like 1 NM_016846 Rgl1 5.28 7.92 0.67
    Leucine rich repeat containing 25 NM_153074 Lrrc25 2.92 4.6 0.64
    Transmembrane protein 176B NM_023056 1810009M01Rik 3.26 5.18 0.63
    Unknown ENSMUST00000094405 ENSMUST00000094405 4.02 6.52 0.62
    Transformed mouse 3T3 cell double minute 4 NM_008575 Mdm4 3.24 5.49 0.59
    C-type lectin domain family 4, member a3 AK156040 Clec4a3 2.92 6.68 0.44
    Predicted gene, OTTMUSG00000000971 BC089618 BC089618 2.92 7.86 0.37
    CD244 natural killer cell receptor 2B4 NM_018729 Cd244 8.25 25.88 0.32
    C-type lectin domain family 4, member b1 NM_027218 Clec4b1 3.06 9.47 0.32
a

A corrected one-way analysis of variance was used to analyze the microarray data. Genes whose expression levels were upregulated at least 2-fold (P < 0.05) compared to unstimulated cells were considered to be differentially expressed in a statistically significant manner. The underlined genes are common to both the 4- and 24-h time points.

b

Bb, B. burgdorferi spirochetes.