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. 2011 Dec;79(12):4868–4875. doi: 10.1128/IAI.05701-11

Fig. 2.

Fig. 2.

Detection of products of the prcB-prcA-prtP operon in T. denticola and purified protease complex. (A) Silver-stained SDS-polyacrylamide gel of purified protease complex (0.4 μg per lane). Lanes: 1, molecular mass standards (kDa); 2, unheated protease complex; 3, boiled protease complex. The locations of the PrtP, PrcA1, and PrcA2 bands are indicated, as is that of the putative PrcB band. (B) Immunoblots of T. denticola whole-cell extracts (equivalent to 10 μl culture per lane) and purified protease complex (0.4 μg per lane) probed with antibodies specific for PrcB, PrcA1, PrcA2, PrtP N-terminal domain, and mature PrtP. The approximate size of each polypeptide (in kDa) is indicated.