IL-1β and IL-18 secretion is dependent on components of the inflammasome complex. (A) BMM from C57BL/6, caspase-1−/−, ASC−/−, and NLRP3−/− mice were infected with the wt or hip1 mutant strain at an MOI of 10. Supernatants collected at 24 h postinfection were assayed for IL-1β and TNF-α by ELISA. BMM from C57BL/6 or TLR2−/− mice (B) or from C57BL/6, caspase-1−/−, ASC−/−, and NLRP3−/− mice (C) were also infected with the wt or hip1 mutant strain at an MOI of 10. Supernatants collected at 24 h postinfection were assayed for IL-18 by ELISA. Values are presented as means plus SD, and the data are representative of 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.