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. 2011 Dec;79(12):4828–4838. doi: 10.1128/IAI.05574-11

Fig. 7.

Fig. 7.

hip1 mutant-induced proinflammatory response is dominant over wt response and is independent of phagocytosis. (A) C57BL/6 BMM were infected with heat-killed (HK) cultures of the wt or the hip1 mutant at an MOI of 10 or 5 or with mixed cultures of the wt and the hip1 mutant at a 1:1 ratio (MOIs of 10 and 10 or 5 and 5). Supernatants collected at 24 h postinfection were assayed for IL-6 by ELISA. (B) C57BL/6 BMM were left untreated or treated with 10 μM cytochalasin D for 1 h and then infected with the heat-killed wt or hip1 mutant strain, and supernatants collected at 6 h postinfection were assayed for IL-6 by ELISA. C57BL/6 (C) or TLR2−/− (D) BMM were left untreated or treated with 10 μM cytochalasin D for 1 h and then infected with the live wt or hip1 mutant strain, and supernatants collected at 24 h postinfection were assayed for IL-6 by ELISA. Values are presented as means plus SD, and the data are representative of 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.