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. 2011 Dec;79(12):5010–5018. doi: 10.1128/IAI.05571-11

Fig. 4.

Fig. 4.

Effect of Treg cells on development of chronic infection in C57BL/6 versus BALB/c mice. (A) Draining lymph nodes were removed from infected C57BL/6 and BALB/c mice at days 7 and 21 postimplantation. Single-cell suspensions were stained intracellularly for Foxp3 as described in Materials and Methods. The Treg cell frequency, expressed as the percentage of Foxp3 expression in CD4+ lymphocytes, was significantly lower for infected C57BL/6 mice than for BALB/c mice at both 7 and 21 days postimplantation. (B) BALB/c mice were treated by i.p. injection of the anti-CD25 MAb PC61.5.3 or an isotype control before implantation of an S. aureus-coated pin and at days 7 and 14 postimplantation, as described in Materials and Methods. Treatment with anti-CD25 reversed the protection against chronic implant infection normally observed in BALB/c mice. A single experiment was performed (n = 8 to 13 mice per group). *, P < 0.05 compared to controls (by Student's t test or Fisher's exact test). Bars represent SD.