Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Description | Source (reference) |
|---|---|---|
| Plasmids | ||
| pRE112 | sacB mobRP4 R6K ori Cmr | (10) |
| pYA4284 | pagL7 deletion | pRE112 |
| pYA4288 | pagP8 deletion | pRE112 |
| pYA4287 | lpxR9 deletion | pRE112 |
| pYA4876 | PagP promoter inserted into pGOA1193 | pGOA1193 (42) |
| pYA3493 | Plasmid Asd+; pBRori β-lactamase signal sequence-based periplasmic secretion plasmid | (25) |
| pYA4088 | 852-bp DNA encoding the α-helical region of PspA from amino acid 3 to 285 in pYA3493 | (62) |
| S. enterica serovar Typhimurium strains | ||
| χ3761 | Wild type, UK-1 | (19) |
| χ8573 | ΔmsbB48 | χ3761 (15) |
| χ11165 | ΔmsbB48ΔpagL7 | χ8573 |
| χ9908 | ΔmsbB48 ΔpagP8 | χ8573 |
| χ9949 | ΔmsbB48 ΔlpxR9 | χ8573 |
| χ11065 | ΔmsbB48 ΔpagL7 ΔpagP8 ΔlpxR9 | χ3761 |
| χ11164 | ΔmsbB48 ΔarnT6 | χ8573 |
| Vaccine strains | ||
| χ9241 | ΔpabA1516 ΔpabB232 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBADlacI TT | (62) |
| χ9278 | ΔpabA1516ΔpabB232 ΔasdA16 ΔaraBAD23 ΔrelA198::araC PBADlacI TT ΔmsbB48 | χ9241 |
| χ11318 | ΔpagL7 | χ9278 |
| χ9848 | ΔpagP8 | χ9278 |
| χ9850 | ΔlpxR9 | χ9278 |
| χ11088 | ΔpagL7 ΔpagP8 ΔlpxR9 | χ9278 |
| E. coli strains | ||
| χ7232 (DH5α λ pir, MGN-026e) | endA1 hsdR17 (rK− mK+) glnV44 thi-1 recA1 gyrA relA1Δ(lacZYA-argF)U169λpirdeoR [φ80dlac Δ(lacZ)M15] | (50) |
| χ7213 (MGN-617) | thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpir ΔasdA4 Δzhf-2::Tn10 | (50) |
| S. pneumoniae WU2 | Wild type, virulent, encapsulated type 3 | (6) |