Fig. 5.

Expression of integrin molecules on human macrophages after treatment with 1 nM CCL1 or 10 ng/ml LPS. (A to F) Expression of CD11b (A), CD18 (B), CD29 (C), CD49b (D), CD49d (E), and CD49f (F) in M-CSF- or GM-CSF-derived macrophages. Expression is shown as geometric mean fluorescence intensity. (G) Results from one representative experiment to show the gating strategy. Viability was monitored in all samples and was routinely >95% (data not shown). The standard deviation was routinely less than 20%. Values are means from three independent donors. Data are from one representative out of three experiments.