Table 1.

Primers used for cloning and expressing P28 constructs of E. muris

P28 paralog	Sequences of primer pairs for PCR and cloning into the following vector:
	pET102/D expression vector	pcDNA3.1CT/GFP vector
P28-9	5′-CACCATGGGAGGGCAATACAG-3	5′-GCCATGGGAGGGCAATACAG-3′
	5′-AACCTCGCCACCAAAGTATCC-3′	5′-CCTCGCCACCAAAGTATCC-3′
P28-12	5′-CACCTCTGGATTATACATTAGCGGACAA-3′	5′-GCCATGGGATTATACATTAGCGGACAA-3′
	5′-ACCAAAGTATCCAACATCAAGTG-3′	5′-CAAAGTATCCAACATCAAGTG-3′
P28-14	5′-CACCGTAGGGAGCGCATTAAT-3′	5′-GCCATGGTAGGGAGCGCA-3′
	5′-TCCAATTTCTCCACCAAAGTAT-3′	5′-CTCCAATTTCTCCACCAAAGTATC-3′
P28-19	5′-CACCGCTTCTCACTTTGGAGTATTTTCAGC-3′	5′-GCCATGGCTTCTCACTTTGGAGTATTTTCAGC-3′
	5′-CCTTCCTCCAAGTTCTATACCAAAATG-3′	5′-TTCCTCCAAGTTCTATACCAAAATG-3′