Table 1.
Primer and protein information
| Antigen | Primer | Molecular mass (kDa) | Vector |
|---|---|---|---|
| rLIC10191 | F CGGGATCCTCAACGCAAGAGCA | 22.9 | pAE |
| R GGGGTACCTTGTTGTGGTGCGGA | |||
| rLIC12099 | F TTGGTACCGCTCAAACGGCAAG | 52.7 | pAE |
| R GGGAAGCTTTTTATATTTGACGATGA | |||
| rLIC11947 | F CCGGATCCCCTGTGGAAAGAAA | 17.9 | pAE |
| R GGGAAGCTTTTTTTCTGGAGGAA | |||
| rLIC10011 | F TAGGTACCACGGATGGACTTTTGAA | 19.8 | pAE |
| R CGGAATTCTTATTGTTTGGAAACCTC | |||
| rLIC12730 | F GCGGATCCATTTTAGTCTTTACCTC | 57.8 | pAE |
| R CCCAAGCTTGATCAATTCCGTTC | |||
| rLIC10561 | F GCGGATCCTTAATATTTCTGGTCTTTC | 30.22 | pAE |
| R CCCAAGCTTGATCAATTCCGTTC | |||
| rLIC10508 | F CGGGATCCAATTCAATAACTATG | 22.99 | pAE |
| R GGGAAGCTTACAACCAGGACCTT | |||
| rLIC12538 | F GGGATCCGCAGACGAAAAGGAAA | 24.9 | pAE |
| R CCAAGCTTTCAGCTAGTCAGAGTAAAA | |||
| rLIC10501 | F CACCGATAACAAAGAGAAGGGAGG | 48.8 | pET100-D/TOPO |
| R CTACTCCACACATTCGGGACTATTG | |||
| rLIC13006 | F GACTCGAGAACTCTGCTTTAAGTGGCTTAA | 47 | pAE |
| R GGCCATGGTTATTGTTCTACACAAACTAAA | |||
| rLIC13306a | F CACCTCCAAAGAGAAATGTTTATTC | 17.7 | pET100-D/TOPO |
| R TCATTTCCGAACCGGATGACCGT | |||
| rLIC12253 | F TTCTCGAGGAGAAACCGGACGATACTACTT | 23.4 | pAE |
| R CCCCATGGTTAGGGAAGACTTCTAACAAC | |||
| rLIC11184 | F CACCTGTGAAGATGAAAAAAAGGA | 18.8 | pET100-D/TOPO |
| R TTAGTAACCACACTCACTCGCAGC | |||
| rLIC10645 | F CACCAAAAAAGATAAGGACGATACCTT | 42.3 | pAE |
| R TTAACGAACTAGTACAGTCGGTAAATG | |||
| rLIC10021 | F GACTCGAGAATTGTTCTGTCAAGCCC | 63.8 | pAE |
| R CCAAGCTTTCATAAATCCACGGAAGT | |||
| rLIC11859a | F CACCGAATTTATGAAGGTCACG | 30 | pET100-D/TOPO |
| R TTAAAAAGCACTTAAGGCAGCC | |||
| rLIC10325a | F CACCATTCAAGACGAAGATTCCAAAC | 40.6 | pET100-D/TOPO |
| R TCAATCCAATTTTTCGGTTTCTAG | |||
| rLIC12555 | F CATCTCGAGAGCCCAGTACAAATGAAAGT | 43 | pAE |
| R TCCATGGTTAAAGATTTGTAACGCAGATTCC | |||
| rLIC11087 | F CACCGTTGGAGATTCCAGAAAGGAA | 29.9 | pET100-D/TOPO |
| R TTAAAATAAATTACAACCAGTCTGATATAA | |||
| rLIC12632 | F GTCTCGAGTGTAAACCTGGCAAACAAAATT | 63.8 | pAE |
| R GGCCATGGCTAATGATGATAGATTAAATCT | |||
| rLIC10054 | F TTTTTTGGATCCGAGTCTAAACGAAG | 32.1 | pQE30 |
| R TTTTTTAAGCTTCACCGTATTCTTGTC | |||
| rLIC20172 | F CGGGATCCGATACGGACAAGGACGGG | 28.1 | pQE30 |
| R CCCAAGCTTTTCGGAATCCTCGTCCGG | |||
| rLIC13059 | F CGGGATCCGAATCCATGGTATATTAT | 14.4 | pQE30 |
| R CCCAAGCTTACTTTGACGAATCAATGC | |||
| rLIC11567 | F CGGGATCCAACAGATTGATTCGTAAA | 13.9 | pQE30 |
| R CCCAAGCTTCTTTTTGTATTCCACAAG | |||
| rLIC10091 | F CGGGATCCAAACTATTTTTAGCTCCTTTG | 14.6 | pQE30 |
| R CCCAAGCTTGATTTCAAAAGAAGTATG | |||
| rLIC10009 | F TTTTTTTGATCACAAGAAGCGCAGATCT | 24.8 | pQE30 |
| R CCCAAGCTTGAACTCATCCTGTTTA | |||
| rLIC13305 | F TTTTTTTGATCAAACTATGATCGTGAC | 20.3 | pQE30 |
| R TTTAAGCTTGATATCACCACCCAAA |
a
Expressed as soluble protein and did not require the use of urea for purification.