Fig. 2.

PCR and Southern blot analyses of genomic DNA extracts from transgenic plants. (a) Gene-specific primers were used to amplify a 602-bp fragment. The G1/G2 gene was present in seven lines of tobacco plants (lanes 1 to 7, right) and four lines of hairy root (lanes 1 to 4, left). (b) The corresponding genomic DNA was examined by Southern blotting using a 602-bp gene-specific probe on tobacco plant lines (lanes 1 to 7, right) and hairy root lines (lanes 1 to 4, left). PC, positive control; WT, wild-type genome as a negative control; Lad, 100-bp DNA ladder.