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(A) Map of pALM33 and sequence of the introduced artificial promoter. The −35 and −10 boxes, Shine-Dalgarno sequence, and start codon are indicated, as well as the transcription start site. The AadA CDS expressed in Chlamydomonas and E. coli are indicated by Cr-AadA and Ec-AadA, respectively. (B) Transformation of Chlamydomonas petC-Δ1 by pALM33. The plasmid was digested by the indicated enzymes, releasing CrEcAadA flanked by the indicated functional elements.
