C. albicans utilizes GroPIns and GroPCho as sources of phosphate. Strains pregrown in 200 μM KH2PO4 (low Pi)-containing medium were harvested, washed, and spotted in a series of 10× dilutions onto plates containing 200 μM GroPIns (A), 200 μM GroPCho (B), 200 μM KH2PO4 (C), or medium containing no phosphate (D). Data are representative of multiple experiments.