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. 2011 Dec;10(12):1618–1627. doi: 10.1128/EC.05160-11

Fig. 8.

Fig. 8.

Effect of PHO4 deletion on growth and transport activity. (A) The wild-type strain and a pho4Δ/Δ deletion mutant were spotted in a series of 10× dilutions onto plates containing the indicated sources of phosphate at a concentration of 200 μM. A second pho4Δ/Δ isolate gave identical results. (B) Uptake activities of the wild type and the two pho4Δ/Δ deletion mutant isolates. Values represent means ± SE of triplicate determinations. The experiment was repeated with similar results. (C) Expression of CaGIT1 in the pho4Δ/Δ mutant and reference wild-type strain. Strains were grown on synthetic YNB supplemented with 200 μM (low Pi) or 10 mM (high Pi) KH2PO4. RNA was extracted and expression measured by quantitative real-time PCR analysis. Values were normalized to the CaTDH3 control and to 1. Values represent the means of triplicate determinations ± SE, where applicable.