Fig. 2.
BPK1 and MCP4 are induced by bradyzoites. HFF monolayers were infected with the PRUΔhxgprt strain of parasites that had been engineered to express a BPK1 or MCP4 protein fused to a C-terminal HA tag (BPK-HA and MCP4-HA, respectively) for 2 days under tachyzoite and 2 days and 4 days under bradyzoite growth conditions. Parasites were released from cells by syringe lysis and counted before SDS-PAGE loading buffer was added. A total of 5 × 105 parasite equivalents were added per lane and probed with antibodies specific for HA, SAG1, or SAG2X as described in Materials and Methods. Representative images from at least two independent experiments are shown. The values to the right are molecular sizes in kilodaltons.