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BPK1 and MCP4 localize to the cyst periphery in vivo. Forty-micrometer coronal brain sections from mice infected 40 days previously with parasites expressing BPK1-HA (A) or MCP4-HA (B) were probed for HA (green) and DBA (red) and examined by confocal microscopy as described in Materials and Methods. The corresponding merged images are also shown. Scale bars represent 10 μm.
