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. 2011 Dec;10(12):1637–1647. doi: 10.1128/EC.05182-11

Fig. 5.

Fig. 5.

BPK1 and MCP4 localize to the cyst wall. EM of tissue cysts stained for BPK1-HA (A and B) and for MCP4-HA (C) using an anti-HA antibody and visualized with 10-nm gold particles. (A) Low-power magnification of a small in vitro tissue cyst (5 dpi) containing bradyzoites (Br) showing numerous gold particles over the cyst wall (CW). Bar represents 1 μm. (B) Detail of the periphery of a 5-day in vitro tissue cyst labeled for BPK1 illustrating the numerous gold particles associated with the cyst wall. Bar represents 100 nm. (C) Detail of the periphery of an in vivo-derived tissue cyst (40 dpi) labeled with MCP4 showing the gold particles specifically associated with the cyst wall. Bar represents 100 nm.