Table 2.
Known bradyzoite and tachyzoite developmentally regulated genes
| Probe set | Gene ID | Gene name | Product | Avg expressiona |
Fold changeb |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2dT | 4dB | 8dB | 21dB | 4dB vs 2dT | 8dB vs 2dT | 21dB vs 2dT | 21dB vs 4dB | ||||
| Upregulated in bradyzoites versus tachyzoites | |||||||||||
| 55.m00009_at | TGME49_059020 | BAG1 | Heat shock protein, bradyzoite antigen | 6.2 | 9.9 | 9.4 | 10.0 | 29 | 18 | 34 | — |
| 59.m03411_at | TGME49_068860 | ENO1 | Enolase 1 | 5.2 | 8.6 | 8.5 | 9.3 | 18 | 16 | 36 | 2.0 |
| 583.m09133_at | TGME49_114250 | BRP1 | Bradyzoite rhoptry protein | 5.0 | 8.2 | 7.6 | 8.8 | 13 | 7.2 | 23 | — |
| 23.m00149_s_at | TGME49_007130 | SAG2Y | Surface antigen (SRS49A) | 4.8 | 6.2 | 6.0 | 7.2 | 2.3 | 2.0 | 5.4 | 2.3 |
| 641.m01562_at | TGME49_120190 | SRS9 | Surface antigen (SRS16B) | 4.1 | 5.5 | 5.6 | 5.6 | 2.0 | 2.0 | 2.1 | — |
| 80.m00010_at | TGME49_091040 | LDH2 | Lactate dehydrogenase 2 | 4.9 | 7.8 | 7.7 | 9.2 | 9.1 | 8.5 | 34 | 3.8 |
| Downregulated in bradyzoites versus tachyzoites | |||||||||||
| 59.m03410_at | TGME49_068850 | ENO2 | Enolase 2 | 7.3 | 4.6 | 5.3 | 4.2 | 0.15 | 0.21 | 0.13 | — |
| 59.m00008_at | TGME49_071050 | SAG2A | Surface antigen (SRS34A, P22) | 8.4 | 6.9 | 7.2 | 6.0 | 0.24 | — | 0.12 | — |
| 44.m00010_at | TGME49_033480 | SRS2 | Surface antigen (SRS29C, P35) | 7.6 | 5.0 | 5.3 | 4.2 | 0.14 | 0.16 | 0.10 | — |
| 44.m00009_at | TGME49_033460 | SAG1 | Surface antigen (SRS29B, P30) | 9.3 | 5.9 | 7.1 | 4.6 | 0.05 | 0.12 | 0.02 | — |
| 44.m00006_at | TGME49_032350 | LDH1 | Lactate dehydrogenase 1 | 6.0 | 5.4 | 5.5 | 5.1 | — | — | 0.55 | — |
a
4dB, 4-dpi bradyzoites; 8dB, 8-dpi bradyzoites; 21dB, 21-dpi bradyzoites; 2dB, 2-dpi tachyzoites. Average normalized glog-transformed expression values.
b
Fold change calculated from glog mean expression values back-transformed to the original scale (see Materials and Methods) and shown only where values are significantly different (P < 0.05). —, fold change not significant.