Fig. 6.

Addition of cAMP restores the dcc-1 mutant phenotypes. (A) The wild-type (wt; left) and dcc-1 mutant (right) strains were inoculated into VGM with or without 2 mM cAMP. After 36 h of incubation in darkness, the plates were illuminated for 24 h. The images presented are representative of the growth of both strains in glucose-rich medium with or without cAMP. (B) The dcc-1 deletion mutant was grown in darkness on VGM plates with or without cAMP. Conidia were harvested and counted at the times indicated. The data, expressed as spores/ml, represent the average of three independent assays, and the standard deviation is depicted. (C) Cultures of the wild-type and dcc-1 mutant strains were grown in standing liquid VSM cultures with or without 2 mM cAMP. After 3 days of incubation at 30°C in darkness, tubes were illuminated for 24 h and photographed. (D) Duplicate cultures of the wild-type and dcc-1 mutant strains were inoculated into liquid VSM with or without 2 mM cAMP. After incubation for 36 h in darkness, one of the cultures was illuminated for 24 h while the other one was kept in darkness. Carotenoids were extracted from 0.1 g of mycelia, and the absorbance at 475 nm was determined. The data, expressed as ng carotenes/mg protein, represent the average of three independent experiments.