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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 19;50(45):9857–9864. doi: 10.1021/bi201498y

Figure 4. Effects of point mutations of NKCC1 amino acids 806-814 on dimerization.

Figure 4

A. Comparison of NKCC1 amino acids 806-814 to the corresponding residues of NKCC2. B and C. Co-immunoprecipitation of nttNKCC1 with the mutants indicated was determined and quantitated as described in the caption of Fig. 1. In the mutants GH-SE, GH-CE and GH-CC both G812 and H813 were mutated as indicated. NCCct, a chimera in which the C-terminus of NKCC1 was replaced with that of NCC, has been described previously (19). Each data point represents the mean ± S.E. for 3 or more independent experiments except M809C, G812T and GH-CE for which n=2.