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. Author manuscript; available in PMC: 2012 Nov 15.
Published in final edited form as: Biochemistry. 2011 Oct 19;50(45):9857–9864. doi: 10.1021/bi201498y

Figure 8. Effect of the sense peptide LMISGHVHMG on the NKCC1 dimer.

Figure 8

Membranes from HEK293 cells transiently transfected with NKCC1 were prepared, solubilized in 0.3 Triton X-100 in the presence or absence of 0.01% SDS and 1 mM of the sense peptide LMISGHVHMG (P) or the scrambled peptide GIHMLGVSMH (SP) as indicated, then treated with the crosslinker DTSSP (1 mM). Peptides were added 1 hour before the crosslinker. The resulting material was probed by Western blotting to determine the amount of crosslinked dimer in each sample. Results were normalized to the amount of dimer observed in the absence of both SDS and peptide. The results of 3 independent experiments were averaged to produce the figure.